EMDB EMD-4369

Single particle reconstruction
3.1Å resolution

Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit.

Map released:
Last modified:
Overview of EMD-4369
Source organisms:
Fitted atomic model: 6gaz
Related EM entry by publication: EMD-4368, EMD-4370
Primary publication:
Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
Kummer E, Leibundgut M, Rackham O, Lee RG, Boehringer D, Filipovska A, Ban N
Nature 560 263-267 (2018)
PMID: 30089917

Function and Biology Details

Sample name: mammalian mitochondrial translation initiation complex
Ligands: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, MAGNESIUM ION, SODIUM ION, SPERMINE, ZINC ION, N-FORMYLMETHIONINE, GUANOSINE-5'-TRIPHOSPHATE, water
Nucleic acids: 12S ribosomal RNA, mitochondrial, P-site fMet-tRNAMet, mitochondrial, MT-CO3 mRNA, mitochondrial
Proteins: mammalian mitochondrial translation initiation complex, Porcine Ribosome, Translation initiation factor IF-2, mitochondrial, Nucleic acids, Translation initiation factor IF-2, mitochondrial, Mitochondrial ribosomal protein L19, Mitochondrial ribosomal protein S2, Mitochondrial ribosomal protein S24, Mitochondrial ribosomal protein S5, Mitochondrial ribosomal protein S6, Mitochondrial ribosomal protein S7, Mitochondrial ribosomal protein S9, Mitochondrial ribosomal protein S10, Mitochondrial ribosomal protein S11, Mitochondrial ribosomal protein S12, Mitochondrial ribosomal protein S14, Mitochondrial ribosomal protein S15, bS16m, MRPS16, Mitochondrial ribosomal protein S17, Mitochondrial ribosomal protein S18C, Mitochondrial ribosomal protein S21, unassigned secondary structure elements, Mitochondrial ribosomal protein S22, Mitochondrial ribosomal protein S23, Mitochondrial ribosomal protein S25, Mitochondrial ribosomal protein S26, Mitochondrial ribosomal protein S27, Mitoribosomal protein ms28, mrps28, Death associated protein 3, mS31, MRPS31, Mitochondrial ribosomal protein S33, Mitochondrial ribosomal protein S34, Mitochondrial ribosomal protein S35, Mitochondrial ribosomal protein S37, Aurora kinase A interacting protein 1, Mitochondrial ribosomal protein S39, 28S ribosomal protein S18b, mitochondrial

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)