EMD-4327

Single-particle
3.5 Å
EMD-4327
3D View Gallery
Deposition: 12/03/2018
Map released: 05/12/2018
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4327
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EMICSS annotations (xml)

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map C1)

EMD-4327

Single-particle
3.5 Å
EMD-4327
3D View Gallery
Deposition: 12/03/2018
Map released: 05/12/2018
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
Complex [6]
Name: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
Molecular weight
Experimental Theoretical Method
- 1 MDa -
Name: Ribosome
Natural source [1]
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Name: tRNA
Natural source [1]
Organism: Saccharomyces cerevisiae
Name: Initiation factors
Natural source [1]
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Name: Initialtion factors
Natural source [1]
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Complex Portal [1]
ID Name Overlap
CPX-1831 Eukaryotic translation initiation factor 3 core complex 0.62
Name: mRNA
Natural source [1]
Organism: Saccharomyces cerevisiae
RNA [3]
Name: tRNAi
Number of copies: 1
Natural source
Organism: Saccharomyces cerevisiae
Molecular weight
Experimental Theoretical Method
- 24 kDa -
Name: 18S ribosomal RNA
Number of copies: 1
Details: RRNA modifications taken from: http://people.biochem.umass.edu/fournierlab/3dmodmap/ysssu2dframes43d.php
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 579 kDa -
Name: mRNA (31-MER)
Number of copies: 1
Natural source
Organism: Saccharomyces cerevisiae
Molecular weight
Experimental Theoretical Method
- 15 kDa -
Protein [44]
LEVO
Name: 40S ribosomal protein S0
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
ribosomal small subunit assembly translation
Molecular function:
structural constituent of ribosome
Cellular location:
cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 28 kDa -
LEVO
Name: 40S ribosomal protein S1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [3]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 29 kDa -
LEVO
Name: KLLA0F09812p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [9]
Biological process:
rRNA export from nucleus translation positive regulation of translational fidelity
Molecular function:
RNA binding structural constituent of ribosome small ribosomal subunit rRNA binding
Cellular location:
small ribosomal subunit small-subunit processome cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 27 kDa -
LEVO
Name: KLLA0D08305p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
translation rRNA export from nucleus ribosomal small subunit export from nucleus cytoplasmic translation
Molecular function:
RNA binding DNA-(apurinic or apyrimidinic site) endonuclease activity structural constituent of ribosome protein kinase A catalytic subunit binding
Cellular location:
small ribosomal subunit cytosolic ribosome cytoplasm cytosolic small ribosomal subunit preribosome, small subunit precursor
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 26 kDa -
LEVO
Name: 40S ribosomal protein S4
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [6]
Biological process:
translation
Molecular function:
structural constituent of ribosome rRNA binding
Cellular location:
ribonucleoprotein complex ribosome protein-containing complex
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 29 kDa -
LEVO
Name: KLLA0D10659p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [6]
Biological process:
translation rRNA export from nucleus
Molecular function:
structural constituent of ribosome RNA binding
Cellular location:
small ribosomal subunit cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 25 kDa -
LEVO
Name: 40S ribosomal protein S6
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 26 kDa -
LEVO
Name: 40S ribosomal protein S7
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 21 kDa -
LEVO
Name: 40S ribosomal protein S8
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 22 kDa -
LEVO
Name: KLLA0E23673p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
rRNA binding structural constituent of ribosome
Cellular location:
small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 21 kDa -
LEVO
Name: KLLA0B08173p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [1]


Cellular location:
cytoplasm
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 12 kDa -
LEVO
Name: KLLA0A10483p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 17 kDa -
LEVO
Name: 40S ribosomal protein S12
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 14 kDa -
LEVO
Name: KLLA0F18040p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
translation maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Molecular function:
structural constituent of ribosome small ribosomal subunit rRNA binding
Cellular location:
ribosome ribonucleoprotein complex cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 16 kDa -
LEVO
Name: 40S ribosomal protein S14
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 14 kDa -
LEVO
Name: KLLA0F07843p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [6]
Biological process:
translation rRNA export from nucleus
Molecular function:
structural constituent of ribosome RNA binding
Cellular location:
small ribosomal subunit cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 15 kDa -
LEVO
Name: 40S ribosomal protein S16
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 15 kDa -
LEVO
Name: KLLA0B01474p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 15 kDa -
LEVO
Name: KLLA0B01562p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
RNA binding structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 17 kDa -
LEVO
Name: KLLA0A07194p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 15 kDa -
LEVO
Name: KLLA0F25542p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) translation
Molecular function:
structural constituent of ribosome
Cellular location:
small ribosomal subunit cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 13 kDa -
LEVO
Name: 40S ribosomal protein S21
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [6]
Biological process:
translation rRNA processing
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome cytoplasm
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 9 kDa -
LEVO
Name: 40S ribosomal protein S22
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 14 kDa -
LEVO
Name: KLLA0B11231p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [3]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 16 kDa -
LEVO
Name: 40S ribosomal protein S24
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex cytosol ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 15 kDa -
LEVO
Name: KLLA0B06182p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 12 kDa -
LEVO
Name: 40S ribosomal protein S26
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 13 kDa -
LEVO
Name: 40S ribosomal protein S27
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
metal ion binding structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 8 kDa -
LEVO
Name: 40S ribosomal protein S28
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay rRNA export from nucleus translation
Molecular function:
structural constituent of ribosome
Cellular location:
ribonucleoprotein complex ribosome cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 7 kDa -
LEVO
Name: 40S ribosomal protein S29
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
structural constituent of ribosome zinc ion binding
Cellular location:
ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 6 kDa -
LEVO
Name: 40S ribosomal protein S30
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [5]
Biological process:
translation
Molecular function:
structural constituent of ribosome
Cellular location:
cytosol ribonucleoprotein complex ribosome
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 7 kDa -
LEVO
Name: Ubiquitin-40S ribosomal protein S27a
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
translation maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) ribosomal small subunit assembly
Molecular function:
structural constituent of ribosome metal ion binding protein tag
Cellular location:
cytoplasm ribonucleoprotein complex ribosome nucleus cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 17 kDa -
LEVO
Name: KLLA0E12277p
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [20]
Biological process:
negative regulation of translational frameshifting negative regulation of cell wall integrity MAPK cascade negative regulation of glucose mediated signaling pathway positive regulation of conjugation with cellular fusion mRNA destabilization positive regulation of autophagy regulation of cytoplasmic translation positive regulation of protein phosphorylation protein localization ribosome-associated ubiquitin-dependent protein catabolic process negative regulation of p38MAPK cascade G protein-coupled receptor signaling pathway invasive growth in response to glucose limitation GCN2-mediated signaling regulation of fungal-type cell wall biogenesis
Molecular function:
translation regulator activity GDP-dissociation inhibitor activity ribosome binding G-protein alpha-subunit binding
Cellular location:
cytosolic small ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 35 kDa -
LEVO
Name: 60S ribosomal protein L41-A
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
cytoplasmic translation
Molecular function:
structural constituent of ribosome
Cellular location:
cytosol cytosolic large ribosomal subunit
Natural source
Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Molecular weight
Experimental Theoretical Method
- 3 kDa -
LEVO
Name: Eukaryotic translation initiation factor 1A
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
translation reinitiation translational initiation formation of translation initiation ternary complex formation of translation preinitiation complex formation of cytoplasmic translation initiation complex
Molecular function:
double-stranded RNA binding ribosomal small subunit binding translation initiation factor binding translation initiation factor activity protein kinase binding
Cellular location:
eukaryotic 43S preinitiation complex eukaryotic 48S preinitiation complex cytoplasmic stress granule cytoplasm
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 17 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: Eukaryotic translation initiation factor 2 subunit alpha
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
translational initiation formation of translation preinitiation complex
Molecular function:
RNA binding translation initiation factor activity ribosome binding
Cellular location:
ribosome cytosol cytoplasmic stress granule eukaryotic translation initiation factor 2B complex cytoplasm multi-eIF complex eukaryotic 48S preinitiation complex eukaryotic translation initiation factor 2 complex
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 34 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 2 subunit gamma
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [15]
Biological process:
positive regulation of translational fidelity formation of translation preinitiation complex translational initiation
Molecular function:
methionyl-initiator methionine tRNA binding translation initiation factor activity GTPase activity GTP binding translation initiation factor binding tRNA binding
Cellular location:
eukaryotic translation initiation factor 2 complex multi-eIF complex eukaryotic 43S preinitiation complex cytosol eukaryotic 48S preinitiation complex ribosome
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 57 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 2 subunit beta
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
translational initiation formation of translation preinitiation complex formation of cytoplasmic translation initiation complex
Molecular function:
metal ion binding translation initiation factor activity mRNA binding translation initiation factor binding
Cellular location:
cytosol multi-eIF complex ribosome eukaryotic 43S preinitiation complex eukaryotic translation initiation factor 2 complex
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 31 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 5
Number of copies: 1
UniProtKB UniProtKB PDBe-KB PDBe-KB AlphaFold DB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
mature ribosome assembly formation of cytoplasmic translation initiation complex negative regulation of translational initiation regulation of translational initiation formation of translation preinitiation complex
Molecular function:
eukaryotic initiation factor eIF2 binding GTP binding translation initiation factor binding GTPase activator activity translation initiation factor activity GDP-dissociation inhibitor activity
Cellular location:
eukaryotic 48S preinitiation complex cytosol multi-eIF complex
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 45 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: Eukaryotic translation initiation factor 3 subunit A
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
translation reinitiation translational initiation formation of cytoplasmic translation initiation complex
Molecular function:
translation initiation factor activity mRNA binding
Cellular location:
eukaryotic translation initiation factor 3 complex, eIF3e eukaryotic 48S preinitiation complex eukaryotic 43S preinitiation complex eukaryotic translation initiation factor 3 complex cytoplasmic stress granule cytoplasm incipient cellular bud site eukaryotic translation initiation factor 3 complex, eIF3m multi-eIF complex
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 110 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 3 subunit B
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [12]
Biological process:
formation of cytoplasmic translation initiation complex translational initiation cytoplasmic translational initiation
Molecular function:
RNA binding translation initiation factor activity identical protein binding translation initiation factor binding
Cellular location:
eukaryotic 48S preinitiation complex eukaryotic translation initiation factor 3 complex eukaryotic 43S preinitiation complex multi-eIF complex cytoplasmic stress granule
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 88 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: eIF3c,eIF3c
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [4]
Biological process:
translational initiation
Molecular function:
translation initiation factor activity translation initiation factor binding
Cellular location:
eukaryotic translation initiation factor 3 complex
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 86 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 3 subunit G
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 30 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
LEVO
Name: Eukaryotic translation initiation factor 3 subunit I
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
positive regulation of ATP-dependent activity formation of cytoplasmic translation initiation complex cytoplasmic translational initiation translational initiation translation reinitiation positive regulation of ATPase activity
Molecular function:
RNA binding translation initiation factor activity
Cellular location:
eukaryotic 43S preinitiation complex eukaryotic translation initiation factor 3 complex, eIF3m eukaryotic 48S preinitiation complex eukaryotic translation initiation factor 3 complex multi-eIF complex cytoplasmic stress granule
Natural source
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Molecular weight
Experimental Theoretical Method
- 38 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Saccharomyces cerevisiae - - -
Ligand [4]
Name: MAGNESIUM ION
HET code: MG
Number of copies: 117
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: ZINC ION
HET code: ZN
Number of copies: 5
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -
Name: METHIONINE
HET code: MET
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 149 Da -
Name: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
HET code: GCP
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 521 Da -