EMDB EMD-4242

Single particle reconstruction
6.3Å resolution

Human cap-dependent 48S pre-initiation complex

Map released:
Last modified:
Overview of EMD-4242
Source organism: Homo sapiens [9606]
Fitted atomic model: 6fec
Related EM entry by publication: EMD-4265
Primary publication:
Structure of a human cap-dependent 48S translation pre-initiation complex.
Eliseev B, Yeramala L, Leitner A, Karuppasamy M, Raimondeau E, Huard K, Alkalaeva E, Aebersold R, Schaffitzel C
Nucleic Acids Res. 46 2678-2689 (2018)
PMID: 29401259

Function and Biology Details

Sample name: human cap-dependent 48S translation pre-initiation complex
Ligand: water
Nucleic acids: 18S ribosomal RNA, Messenger RNA (26-MER), Transfer RNA (75-MER)
Proteins: human cap-dependent 48S translation pre-initiation complex, human cap-dependent 48S translation pre-initiation complex, mRNA, Eukaryotic translation initiation factor 4B, Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit C, Eukaryotic translation initiation factor 3 subunit E, Eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit H, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit L, Eukaryotic translation initiation factor 3 subunit M, EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D, 40S ribosomal protein S11, 40S ribosomal protein S16, 40S ribosomal protein S4, X isoform, 40S ribosomal protein S29, 40S ribosomal protein S9, 40S ribosomal protein S18, Eukaryotic translation initiation factor 2 subunit 1, 40S ribosomal protein S23, 40S ribosomal protein S19, Eukaryotic translation initiation factor 2 subunit 3, 40S ribosomal protein S5, 40S ribosomal protein S30, 40S ribosomal protein S25, 40S ribosomal protein S7, 40S ribosomal protein S27, 40S ribosomal protein S13, 40S ribosomal protein S15a, 40S ribosomal protein S21, 40S ribosomal protein S2, EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT (eIF2-Beta), 40S ribosomal protein S17, 40S ribosomal protein SA, 40S ribosomal protein S3, 40S ribosomal protein S20, 40S ribosomal protein S3a, 40S ribosomal protein S14, 40S ribosomal protein S26, 40S ribosomal protein S28, Receptor of activated protein C kinase 1, 40S ribosomal protein S15, 40S ribosomal protein S8, Ubiquitin-40S ribosomal protein S27a, 40S ribosomal protein S6, 40S ribosomal protein S12, 40S ribosomal protein S24, 40S ribosomal protein S10, Eukaryotic translation initiation factor 4B, Eukaryotic translation initiation factor 3 subunit B

Experimental Information Details

Resolution: 6.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)