EMD-4151

Single-particle
27.0 Å
EMD-4151
3D View Gallery
Deposition: 17/10/2016
Map released: 21/12/2016
Last modified: 02/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4151
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

S.cerevisiae Elp123 sub-complex

EMD-4151

Single-particle
27.0 Å
EMD-4151
3D View Gallery
Deposition: 17/10/2016
Map released: 21/12/2016
Last modified: 02/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.035 mg/mL
Buffer
pH: 7.6
Buffer components [3]:
Name Formula Concentration ChEBI
HEPES buffer C8H18N2O4S 10.0 mM
Sodium chloride NaCl 125.0 mM
2-mercaptoethanol C2H6OS 5.0 mM
Staining
Type: NEGATIVE
Material: Uranyl Acetate
Details: 3.5 ul aliquots of freshly purified Elp123 complex were applied to glow discharged carbon copper-collodion (Sigma) grids for 2 min and stained with a 1% uranyl acetate solution (w/v)
Grid
Mesh: 400
Model: Plano D-35578
Material: COPPER
Details: 0.45mBar, 20mA
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 6.3 mm
Nominal magnification: 49000.0
Alignment procedure: BASIC
Image Recording [1]
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of grids: 1
Number of real images: 216
Average electron dose per image: 16.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 27.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 2051
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: RANDOM CONICAL TILT
Random conical tilt
Number of images: 120
Tilt angle: 55°
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
Xmipp - -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 1.4 -
Particle selection [1]
Selected Ref. model Method Software Details
50034 - - - -
Final 3D classification
Number of classes: 2
Avg. number of members per classes: 3200.0
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Software [1]
Name Version Details
CTFFIND 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Negative staining reconstruction of the S.cerevisiae Elp123 sub-complex
⬡ Geometry
X Y Z
Dimensions 224 224 224
Origin 0 0 0
Spacing 224 224 224
Voxel size 2.2 Å 2.2 Å 2.2 Å
Contour list
Primary Level Source
True 0.027 AUTHOR