EMDB EMD-4055

Single particle reconstruction
3.8Å resolution

Overall map of the yeast spliceosome immediately after branching

Map released:
Last modified:
Overview of EMD-4055
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5lj3
Related EM entries by publication: EMD-4056, EMD-4057, EMD-4058, EMD-4059
Primary publication:
Cryo-EM structure of the spliceosome immediately after branching.
Galej WP, Wilkinson ME, Fica SM, Oubridge C, Newman AJ, Nagai K
Nature 537 197-201 (2016)
PMID: 27459055

Function and Biology Details

Sample name: Spliceosome immediately after branching
Ligands: MAGNESIUM ION, ZINC ION, GUANOSINE-5'-TRIPHOSPHATE
Nucleic acids: U5 snRNA (small nuclear RNA), Exon 1 (5' exon) of UBC4 pre-mRNA, Intron of UBC4 pre-mRNA, U2 snRNA (small nuclear RNA), U6 snRNA (small nuclear RNA)
Proteins: Spliceosome immediately after branching, Pre-mRNA-splicing factor 8, Protein CWC16, Pre-mRNA-splicing factor CWC25, Pre-mRNA-splicing factor SNU114, ISY1, CWC22, PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor BUD31, CWC2, Pre-mRNA-splicing factor SLT11, CEF1, CWC15, Pre-mRNA-splicing factor CWC21, CLF1, SYF1, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', unknown

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)