EMDB EMD-3979

Single particle reconstruction
3.7Å resolution

Post-catalytic P complex spliceosome with 3' splice site docked

Map released:
Last modified:
Overview of EMD-3979
Source organism: Saccharomyces cerevisiae [559292]
Fitted atomic model: 6exn
Related EM entry by publication: EMD-3980
Primary publication:
Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.
Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K
Science 358 1283-1288 (2017)
PMID: 29146871

Function and Biology Details

Sample name: P complex spliceosome with 3' exon docked
Ligands: MAGNESIUM ION, INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, ZINC ION
Nucleic acids: U2 snRNA, U5 snRNA, U6 snRNA, Ligated exons: UBC4 mRNA, Intron lariat: UBC4 RNA
Proteins: P complex spliceosome with 3' exon docked, P complex spliceosome, 3' exon, Pre-mRNA-splicing factor Prp8, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor CWC16, Pre-mRNA-splicing factor CWC22, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor BUD31, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor CLF1, Pre-mRNA-splicing factor SYF1, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, U2 small nuclear ribonucleoprotein A', Unassigned structure, U2 small nuclear ribonucleoprotein B'', Pre-mRNA-splicing factor Prp18, Small nuclear ribonucleoprotein-associated protein B, Pre-mRNA-splicing factor SLU7, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Pre-mRNA-processing factor Prp17, Pre-mRNA-splicing factor SNT309, Pre-mRNA-processing factor Prp19, Pre-mRNA-splicing factor SYF2

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)