EMDB EMD-3908

Single particle reconstruction
3.55Å resolution

PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae

Map released:
Last modified:
Overview of EMD-3908
Fitted atomic model: 6eoj
Primary publication:
Architecture of eukaryotic mRNA 3'-end processing machinery.
Casanal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA
Science 358 1056-1059 (2017)
PMID: 29074584

Function and Biology Details

Sample name: Complex of Cft1, Yth1, Pfs2 and Fip1
Ligand: ZINC ION
Proteins: Complex of Cft1, Yth1, Pfs2 and Fip1, Protein CFT1, mRNA 3'-end-processing protein YTH1, Polyadenylation factor subunit 2,Polyadenylation factor subunit 2

Experimental Information Details

Resolution: 3.55Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)