EMDB EMD-3843

Single particle reconstruction
3.03Å resolution

Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA

Map released:
Last modified:
Overview of EMD-3843
Source organism: Homo sapiens [9606]
Fitted atomic model: 5oom
Related EM entry by publication: EMD-3842
Primary publication:
Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nat. Struct. Mol. Biol. 24 866-869 (2017)
PMID: 28892042

Function and Biology Details

Sample name: Mitochondrial ribosome large subunit with assembly factor
Cellular component: Mitochondrial ribosome large subunit with assembly factor
Ligands: MAGNESIUM ION, ZINC ION, 4'-PHOSPHOPANTETHEINE
Nucleic acids: 16S ribosomal RNA, mitochondrial tRNAVal
Proteins: 39S ribosomal protein L2, mitochondrial, 39S ribosomal protein L3, mitochondrial, 39S ribosomal protein L4, mitochondrial, 39S ribosomal protein L9, mitochondrial, 39S ribosomal protein L10, mitochondrial, 39S ribosomal protein L11, mitochondrial, 39S ribosomal protein L13, mitochondrial, 39S ribosomal protein L14, mitochondrial, 39S ribosomal protein L15, mitochondrial, 39S ribosomal protein L16, mitochondrial, 39S ribosomal protein L17, mitochondrial, 39S ribosomal protein L18, mitochondrial, 39S ribosomal protein L19, mitochondrial, 39S ribosomal protein L20, mitochondrial, 39S ribosomal protein L21, mitochondrial, 39S ribosomal protein L22, mitochondrial, 39S ribosomal protein L23, mitochondrial, 39S ribosomal protein L24, mitochondrial, 39S ribosomal protein L27, mitochondrial, 39S ribosomal protein L28, mitochondrial, 39S ribosomal protein L47, mitochondrial, 39S ribosomal protein L30, mitochondrial, 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L33, mitochondrial, 39S ribosomal protein L34, mitochondrial, 39S ribosomal protein L35, mitochondrial, 39S ribosomal protein L37, mitochondrial, 39S ribosomal protein L38, mitochondrial, 39S ribosomal protein L39, mitochondrial, 39S ribosomal protein L40, mitochondrial, 39S ribosomal protein L41, mitochondrial, 39S ribosomal protein L42, mitochondrial, 39S ribosomal protein L43, mitochondrial, 39S ribosomal protein L44, mitochondrial, 39S ribosomal protein L45, mitochondrial, 39S ribosomal protein L46, mitochondrial, 39S ribosomal protein L48, mitochondrial, 39S ribosomal protein L49, mitochondrial, 39S ribosomal protein L50, mitochondrial, 39S ribosomal protein L51, mitochondrial, 39S ribosomal protein L52, mitochondrial, 39S ribosomal protein L53, mitochondrial, 39S ribosomal protein L54, mitochondrial, 39S ribosomal protein L55, mitochondrial, Ribosomal protein 63, mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, Growth arrest and DNA damage-inducible proteins-interacting protein 1, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S30, mitochondrial, Unknown protein or protein extension, Mitochondrial assembly of ribosomal large subunit protein 1, MIEF1 upstream open reading frame protein, Acyl carrier protein, mitochondrial

Experimental Information Details

Resolution: 3.03Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)