EMDB EMD-3817

Single particle reconstruction
3.7Å resolution

Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC3

Map released:
Last modified:
Overview of EMD-3817
Source organisms:
Fitted atomic model: 5oik
Related in-frame EM entries: EMD-3815, EMD-3816, EMD-3818, EMD-3819
Related EM entries by publication: EMD-3815, EMD-3816, EMD-3818, EMD-3819
Primary publication:
Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
Bernecky C, Plitzko JM, Cramer P
Nat. Struct. Mol. Biol. 24 809-815 (2017)
PMID: 28892040

Function and Biology Details

Sample name: RNA polymerase II-DSIF elongation complex
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (43-MER), RNA (5'-R(P*UP*AP*UP*AP*UP*AP*CP*AP*UP*AP*AP*AP*GP*AP*CP*CP*AP*GP*GP*C)-3'), DNA (43-MER)
Proteins: RNA polymerase II-DSIF elongation complex, RNA polymerase II, DSIF, DNA-RNA synthetic construct, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase II subunit RPB3, Polymerase (RNA) II (DNA directed) polypeptide D, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase II subunit RPB7, Uncharacterized protein, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, DNA-directed RNA polymerases I, II, and III subunit RPABC4, Transcription elongation factor SPT4, Transcription elongation factor SPT5

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)