EMDB EMD-3802

Single particle reconstruction
4.4Å resolution

The cryo-EM structure of human TFIIH

Map released:
Last modified:
Overview of EMD-3802
Source organism: Homo sapiens [9606]
Fitted atomic model: 5of4
Related EM entry by publication: EMD-8816, EMD-8817
Primary publication:
The cryo-electron microscopy structure of human transcription factor IIH.
Greber BJ, Nguyen THD, Fang J, Afonine PV, Adams PD, Nogales E
Nature 549 414-417 (2017)
PMID: 28902838

Function and Biology Details

Sample name: TFIIH
Ligand: IRON/SULFUR CLUSTER
Proteins: TFIIH, TFIIH core complex, TFIIH CDK-activating kinase (CAK) subcomplex, TFIIH basal transcription factor complex helicase XPB subunit,XPB,TFIIH basal transcription factor complex helicase XPB subunit, TFIIH basal transcription factor complex helicase XPD subunit, General transcription factor IIH subunit 4,p52,General transcription factor IIH subunit 4, General transcription factor IIH subunit 2, General transcription factor IIH subunit 3, General transcription factor IIH subunit 5, MAT1, Unassigned secondary structure elements., Unassigned secondary structure elements (p52 region), Unassigned secondary structure elements (XPB NTE region)

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN
Detector: GATAN K2 (4k x 4k)