EMDB EMD-3751

Single particle reconstruction
3.31Å resolution

Cryo-EM reconstruction of the Mycobacterium smegmatis 70S ribosome

Map released:
Last modified:
Overview of EMD-3751
Source organism: Mycobacterium smegmatis str. MC2 155 [246196]
Fitted atomic model: 5o61
Related EM entry by publication: EMD-3748, EMD-3750
Primary publication:
The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N
Cell Rep 20 149-160 (2017)
PMID: 28683309

Function and Biology Details

Sample name: 70S ribosome
Ligands: MAGNESIUM ION, ZINC ION, PHENYLALANINE
Nucleic acids: 23S rRNA, 5S rRNA, 16S rRNA, P/P-site Phe-tRNA(Phe), mRNA fragment
Proteins: 70S ribosome, 50S ribosomal protein bL37, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, LSU ribosomal protein L28P, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33 1, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 50S ribosomal protein L31, Conserved domain protein, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18 2, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S2

Experimental Information Details

Resolution: 3.31Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)