EMDB EMD-3750

Single particle reconstruction
3.18Å resolution

Cryo-EM reconstruction of the large subunit of the Mycobacterium smegmatis ribosome

Map released:
Last modified:
Overview of EMD-3750
Source organism: Mycobacterium smegmatis str. MC2 155 [246196]
Fitted atomic model: 5o60
Related EM entry by publication: EMD-3748, EMD-3751
Primary publication:
The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N
Cell Rep 20 149-160 (2017)
PMID: 28683309

Function and Biology Details

Sample name: 50S large ribosomal subunit
Ligands: MAGNESIUM ION, ZINC ION, PHENYLALANINE
Nucleic acids: 23S rRNA, 5S rRNA, tRNA CCA-end acetylated (Phe)
Proteins: 50S large ribosomal subunit, 50S ribosomal protein bL37, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33 1, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 50S ribosomal protein L31

Experimental Information Details

Resolution: 3.18Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)