EMDB EMD-3748

Single particle reconstruction
3.45Å resolution

Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome

Map released:
Last modified:
Overview of EMD-3748
Source organism: Mycobacterium smegmatis str. MC2 155 [246196]
Fitted atomic model: 5o5j
Related EM entry by publication: EMD-3750, EMD-3751
Primary publication:
The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N
Cell Rep 20 149-160 (2017)
PMID: 28683309

Function and Biology Details

Sample name: 30S small ribosomal subunit
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: 16S rRNA, tRNA-Phe anticodon stem, mRNA fragment
Proteins: 30S small ribosomal subunit, Conserved domain protein, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18 2, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S2, 50S ribosomal protein L31

Experimental Information Details

Resolution: 3.45Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)