EMD-3708

Single-particle
18.0 Å
EMD-3708
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Deposition: 05/05/2017
Map released: 06/09/2017
Last modified: 01/04/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3708
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EMICSS annotations (xml)

Negative-stain surface of SorCS1 monomer

EMD-3708

Single-particle
18.0 Å
EMD-3708
3D View Gallery
Deposition: 05/05/2017
Map released: 06/09/2017
Last modified: 01/04/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Staining
Type: NEGATIVE
Material: Uranyl Formate
Microscopy [1]
Microscope: FEI TECNAI SPIRIT
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Average electron dose per image: 8.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 18.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 34000
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: PDB ENTRY
PDB model: 3F6K
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Negative stain surface of mSorCS1 monomer
⬡ Geometry
X Y Z
Dimensions 28 28 28
Origin -14 -14 -14
Spacing 28 28 28
Voxel size 6.29 Å 6.29 Å 6.29 Å
Contour list
Primary Level Source
True 0.06 AUTHOR