EMD-3656

Single-particle
3.8 Å
EMD-3656
3D View Gallery
Deposition: 29/03/2017
Map released: 14/06/2017
Last modified: 20/02/2019
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EMD-3656
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization.

EMD-3656

Single-particle
3.8 Å
EMD-3656
3D View Gallery
Deposition: 29/03/2017
Map released: 14/06/2017
Last modified: 20/02/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Details: 4 OD260/ml ml Bs100S sample were applied to 2 nm pre-coated Quantifoil R3/3 holey carbon supported grids and vitrified using Vitrobot Mark IV (FEI Company)
Buffer
pH: 7.5
Buffer components [3]:
Name Formula Concentration ChEBI
Hepes-KOH - 50.0 mM -
- KOAc 100.0 mM
- Mg(OAc)2 25.0 mM
Grid
Model: Quantifoil R3/3
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
- HOLEY -
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 2.5 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 24546
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN 9.11 -
Startup model [1]
Type: PDB ENTRY
PDB model: 3JW9
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
FREALIGN 9.11 -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
FREALIGN 9.11 -
Particle selection [1]
Selected Ref. model Method Software Details
253905 - - - -
Final 3D classification
Software [1]
Name Version Details
FREALIGN 9.11 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of the Bacillus subtilis 100s subcomplex (70s-30s)
⬡ Geometry
X Y Z
Dimensions 450 450 450
Origin 0 0 0
Spacing 450 450 450
Voxel size 1.084 Å 1.084 Å 1.084 Å
Contour list
Primary Level Source
True 11.0 AUTHOR