EMD-3622

Single-particle
4.4 Å
EMD-3622
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Deposition: 07/03/2017
Map released: 04/10/2017
Last modified: 04/10/2017
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3622
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EMICSS annotations (xml)

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFx

EMD-3622

Single-particle
4.4 Å
EMD-3622
3D View Gallery
Deposition: 07/03/2017
Map released: 04/10/2017
Last modified: 04/10/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx
Complex [3]
Name: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx
Details: Microtubule bound to taxol
Name: Tubulin alpha and beta chains
Details: Microtubule bound to taxol
Natural source [1]
Organism: Bos taurus
Name: Kinesin-like protein KIF20A
Details: Microtubule bound to taxol
Natural source [1]
Organism: Mus musculus
Recombinant Expression [1]
Organism Strain Cell Plasmid
Escherichia coli - - -
Protein [3]
LEVO
Name: Kinesin-like protein KIF20A
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [7]
Biological process:
microtubule-based movement mitotic cytokinesis
Molecular function:
microtubule motor activity ATP binding microtubule binding
Cellular location:
Golgi apparatus microtubule
Natural source
Organism: Mus musculus
Molecular weight
Experimental Theoretical Method
- 56 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: Tubulin alpha chain
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [14]
Biological process:
cellular response to interleukin-4 microtubule cytoskeleton organization mitotic cell cycle
Molecular function:
ubiquitin protein ligase binding double-stranded RNA binding metal ion binding GTP binding GTPase activity structural constituent of cytoskeleton
Cellular location:
cytoplasmic microtubule microtubule cytoskeleton cytoplasm cytosol microtubule
Natural source
Organism: Bovine
Molecular weight
Experimental Theoretical Method
- 45 kDa -
LEVO
Name: Tubulin beta-2B chain
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
positive regulation of axon guidance microtubule cytoskeleton organization nervous system development mitotic cell cycle microtubule-based process
Molecular function:
metal ion binding GTPase activity GTP binding protein heterodimerization activity structural constituent of cytoskeleton
Cellular location:
microtubule microtubule cytoskeleton cytoplasm
Natural source
Organism: Bovine
Molecular weight
Experimental Theoretical Method
- 47 kDa -
Ligand [6]
Name: ADENOSINE-5'-DIPHOSPHATE
HET code: ADP
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 427 Da -
Name: MAGNESIUM ION
HET code: MG
Number of copies: 2
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: TETRAFLUOROALUMINATE ION
HET code: ALF
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 102 Da -
Name: GUANOSINE-5'-TRIPHOSPHATE
HET code: GTP
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 523 Da -
Name: GUANOSINE-5'-DIPHOSPHATE
HET code: GDP
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 443 Da -
Name: TAXOL
HET code: TA1
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 853 Da -