EMDB EMD-3622

Single particle reconstruction
4.4Å resolution

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFx

Map released:
Overview of EMD-3622
Source organisms:
Fitted atomic model: 5nd4
Related EM entries by publication: EMD-3620, EMD-3621, EMD-3623
Primary publication:
The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry.
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
Elife 6 - (2017)
PMID: 28826477

Function and Biology Details

Sample name: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx
Ligands: ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, TETRAFLUOROALUMINATE ION, GUANOSINE-5'-TRIPHOSPHATE, GUANOSINE-5'-DIPHOSPHATE, TAXOL
Proteins: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx, Tubulin alpha and beta chains, Kinesin-like protein KIF20A, Kinesin-like protein KIF20A, Tubulin alpha chain, Tubulin beta-2B chain

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI POLARA 300
Detector: DIRECT ELECTRON DE-20 (5k x 3k)