EMDB EMD-3620

Single particle reconstruction
5.8Å resolution

Microtubule-bound MKLP2 motor domain in the presence of ADP

Map released:
Overview of EMD-3620
Source organisms:
Fitted atomic model: 5nd2
Related EM entries by publication: EMD-3621, EMD-3622, EMD-3623
Primary publication:
The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry.
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
Elife 6 - (2017)
PMID: 28826477

Function and Biology Details

Sample name: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP
Ligands: ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, GUANOSINE-5'-TRIPHOSPHATE, GUANOSINE-5'-DIPHOSPHATE, TAXOL
Proteins: Complex of 14pf microtubule with bound MKLP2 motor domain in the presence of ADP, Kinesin-like protein KIF20A, Tubulin alpha and beta chains, Kinesin-like protein KIF20A, Tubulin alpha chain, Tubulin beta-2B chain

Experimental Information Details

Resolution: 5.8Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI POLARA 300
Detector: OTHER