EMD-3618

Single-particle
3.6 Å
EMD-3618 Deposition: 07/03/2017
Map released: 14/06/2017
Last modified: 02/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3618

Bypassing 70S ribosome

EMD-3618

Single-particle
3.6 Å
EMD-3618 Deposition: 07/03/2017
Map released: 14/06/2017
Last modified: 02/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Buffer components [6]:
Name Formula Concentration ChEBI
- Tris-HCl 50.0 mM
- NH4Cl 70.0 mM
- KCL 30.0 mM
- MgCl2 3.5 mM
- putrescine 8.0 mM
- spermidine 0.5 mM
Grid
Model: Quantifoid R2/1
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON - -
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Nominal defocus: 0.6 µm - 3.0 µm
Calibrated magnification: 47170.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Frames per image: 2-10
Average electron dose per image: 26.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 37000
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 1.4 -
Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 1.4 -
Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 1.4 -
Particle selection [1]
Selected Ref. model Method Software Details
36984 - - - -
Final 3D classification
Details: Hierarchical 3D classification
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Details:Wiener filter
Software [1]
Name Version Details
CTFFIND - Wiener filtering inside Relion
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Raw map
Geometry
X Y Z
Dimensions 340 340 340
Origin 0 0 0
Spacing 340 340 340
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.0045 AUTHOR