EMD-3618
Bypassing 70S ribosome
EMD-3618
Single-particle3.6 Å
Deposition: 07/03/2017
Map released: 14/06/2017
Last modified: 02/08/2017
Buffer
pH: 7.5
Buffer components [6]:
Buffer components [6]:
Name | Formula | Concentration | ChEBI |
---|---|---|---|
- | Tris-HCl | 50.0 mM | |
- | NH4Cl | 70.0 mM | |
- | KCL | 30.0 mM | |
- | MgCl2 | 3.5 mM | |
- | putrescine | 8.0 mM | |
- | spermidine | 0.5 mM |
Grid
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Nominal defocus: 0.6 µm - 3.0 µm
Calibrated magnification: 47170.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Nominal defocus: 0.6 µm - 3.0 µm
Calibrated magnification: 47170.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Frames per image: 2-10
Average electron dose per image: 26.0 e/Å2
Detector mode: COUNTING
Frames per image: 2-10
Average electron dose per image: 26.0 e/Å2
Final
reconstruction
Resolution: 3.6
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 37000
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 37000
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
⦨ Initial angle
assignment
⦩ Final angle assignment
Particle selection
[1]
Selected | Ref. model | Method | Software | Details |
---|---|---|---|---|
36984 | - | - | - | - |
Final 3D classification
Details:
Hierarchical 3D classification
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
CTF correction
Details:Wiener filter
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | - | Wiener filtering inside Relion |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Raw map
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Raw map
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 340 | 340 | 340 |
Origin | 0 | 0 | 0 |
Spacing | 340 | 340 | 340 |
Voxel size | 1.06 Å | 1.06 Å | 1.06 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.0045 | AUTHOR |