Single particle reconstruction
5.9Å resolution

Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)

Map released:
Last modified:
Overview of EMD-3545
Source organism: Human [9606]
Fitted atomic model: 5mqf
Related EM entry by publication: EMD-3547
Primary publication:
Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lu Hrmann R
Nature 542 318-323 (2017)
PMID: 28076346

Function and Biology Details

Sample name: Human C* Spliceosome, masked refinement
Nucleic acids: MINX pre-mRNA (intron), Human gene for small nuclear RNA U2 (snRNA U2), Homo sapiens U5 A small nuclear RNA, Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA
Proteins: Human C* Spliceosome, masked refinement, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, SNW domain-containing protein 1, Pleiotropic regulator 1, Pre-mRNA-processing factor 17, U5 small nuclear ribonucleoprotein 40 kDa protein, Pre-mRNA-processing factor 19, Pre-mRNA-splicing factor SPF27, Cell division cycle 5-like protein, Pre-mRNA-splicing factor SYF1, Pre-mRNA-splicing factor SYF2, Crooked neck-like protein 1, Pre-mRNA-splicing factor RBM22, Protein BUD31 homolog, Spliceosome-associated protein CWC15 homolog, Serine/arginine repetitive matrix protein 2, Pre-mRNA-splicing factor CWC22 homolog, Intron-binding protein aquarius, Peptidyl-prolyl cis-trans isomerase-like 1, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D1, Peptidyl-prolyl cis-trans isomerase E, Eukaryotic initiation factor 4A-III, ATP-dependent RNA helicase DHX8

Experimental Information Details

Resolution: 5.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Detector: FEI FALCON II (4k x 4k)