EMDB EMD-3541

Single particle reconstruction
4.17Å resolution

Structure of a spliceosome remodeled for exon ligation

Map released:
Last modified:
Overview of EMD-3541
Source organism: Baker's yeast [4932]
Fitted atomic model: 5mq0
Related EM entry by publication: EMD-3539, EMD-3542
Primary publication:
Structure of a spliceosome remodelled for exon ligation.
Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K
Nature 542 377-380 (2017)
PMID: 28076345

Function and Biology Details

Sample name: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling
Ligands: MAGNESIUM ION, POTASSIUM ION, INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, ZINC ION
Nucleic acids: Yeast UBC4 gene for ubiquitin-conjugating enzyme, 5'-EXON OF UBC4 PRE-MRNA, S.cerevisiae chromosome II reading frame ORF YBR230c, Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence, Saccharomyces cerevisiae strain WI_C_MBSP_4 chromosome VII sequence, 3'-EXON OF UBC4 PRE-MRNA, BOUND BY PRP22 HELICASE
Proteins: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling, Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor CWC22, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor BUD31, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor CLF1, Pre-mRNA-splicing factor SYF1,PRE-MRNA-SPLICING FACTOR SYF1, Pre-mRNA-splicing factor 18, Pre-mRNA-splicing factor SLU7, Pre-mRNA-processing factor 17, UNKNOWN PROTEIN, Pre-mRNA-splicing factor SYF2, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', Pre-mRNA-splicing factor SNT309, Pre-mRNA-processing factor 19

Experimental Information Details

Resolution: 4.17Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)