EMDB EMD-3444

Single particle reconstruction
4.8Å resolution

Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins

Map released:
Last modified:
Overview of EMD-3444
Source organisms:
Fitted atomic model: 5m5c
Related EM entry by publication: EMD-4154, EMD-4156
Primary publication:
A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, Akhmanova A
Nat. Struct. Mol. Biol. 24 931-943 (2017)
PMID: 28991265

Function and Biology Details

Sample name: Complex of two tubulin dimers with bound CAMSAP1-N1492A MUTANT CKK domain
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, TAXOL, water
Proteins: Complex of two tubulin dimers with bound CAMSAP1-N1492A MUTANT CKK domain, tubulin dimer, CAMSAP1-N1492A MUTANT CKK domain, Calmodulin-regulated spectrin-associated protein 1, Tubulin alpha chain, Tubulin beta-2B chain

Experimental Information Details

Resolution: 4.8Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI POLARA 300
Detector: GATAN K2 (4k x 4k)