EMD-3376

Single-particle
3.97 Å
EMD-3376 Deposition: 09/03/2016
Map released: 18/05/2016
Last modified: 18/05/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3376

Transcription initiation complex structures elucidate promoter DNA opening (OC2)

EMD-3376

Single-particle
3.97 Å
EMD-3376 Deposition: 09/03/2016
Map released: 18/05/2016
Last modified: 18/05/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: yeast Pol II transcription initiation complex (open DNA)
Sample [1]
Name: yeast Pol II transcription initiation complex (open DNA)
Number of unique components: 7
Oligomeric state: Monomer
Molecular weight
Experimental Theoretical Method
- 890 kDa -
Protein [6]
Name: DNA-directed RNA polymerase II (RNA polymerase II)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 513 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: Transcription Factor IIA (TFIIA)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 45 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3)RIL - pOPINE
Name: Transcription Factor IIB (TFIIB)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 38 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3)RIL - pET21b
Name: TATA-box-binding protein (TBP, SPT15)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 27 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3)RIL - pOPINE
Name: Transcription Factor IIE (TFIIE)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 91 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3)RIL - pET21
Name: Transcription Factor IIF (TFIIF)
Number of copies: 1
Natural source
Molecular weight
Experimental Theoretical Method
- 129 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3)RIL - pETDuet-1
DNA [1]
Name: synthetic promoter DNA construct (open)
Details: Contains a 15 nucleotide mismatch bubble
Natural source
Organism: unidentified
Molecular weight
Experimental Theoretical Method
- 44 kDa -