EMD-3376
Transcription initiation complex structures elucidate promoter DNA opening (OC2)
EMD-3376
Single-particle3.97 Å
Deposition: 09/03/2016
Map released: 18/05/2016
Last modified: 18/05/2016
Name: yeast Pol II transcription initiation complex (open DNA)
Summary:
yeast Pol II transcription initiation complex (open DNA)
Number of unique components: 7
Oligomeric state: Monomer
Name: Number of unique components: 7
Oligomeric state: Monomer
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 890 kDa | - |
DNA-directed RNA polymerase II
(RNA polymerase II)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 513 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
- | - | - | - |
Transcription Factor IIA
(TFIIA)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 45 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL21(DE3)RIL | - | pOPINE |
Transcription Factor IIB
(TFIIB)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 38 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL21(DE3)RIL | - | pET21b |
TATA-box-binding protein
(TBP, SPT15)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 27 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL21(DE3)RIL | - | pOPINE |
Transcription Factor IIE
(TFIIE)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 91 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL21(DE3)RIL | - | pET21 |
Transcription Factor IIF
(TFIIF)
Number of copies: 1
Name: Number of copies: 1
Natural source
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 129 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
Escherichia coli | BL21(DE3)RIL | - | pETDuet-1 |
synthetic promoter DNA construct (open)
Details: Contains a 15 nucleotide mismatch bubble
Name: Details: Contains a 15 nucleotide mismatch bubble
Natural source
Organism: unidentified
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
- | 44 kDa | - |