EMDB EMD-3376

Single particle reconstruction
3.97Å resolution

Transcription initiation complex structures elucidate promoter DNA opening (OC2)

Map released:
Overview of EMD-3376
Source organisms:
Primary publication:
Transcription initiation complex structures elucidate promoter DNA opening
Plaschka C, Hantsche M, Dienemann C, Burzinski C, Plitzko J, Cramer P
NATURE (2016)

Function and Biology Details

Sample name: yeast Pol II transcription initiation complex (open DNA)
Nucleic acid: synthetic promoter DNA construct (open)
Proteins: DNA-directed RNA polymerase II, Transcription Factor IIA, Transcription Factor IIB, TATA-box-binding protein, Transcription Factor IIE, Transcription Factor IIF

Experimental Information Details

Resolution: 3.97Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)