EMD-2984

Single-particle
2.2 Å
EMD-2984
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Deposition: 26/04/2015
Map released: 06/05/2015
Last modified: 14/10/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2984
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5a1a summary report (pdf.gz) Map and 5a1a FULL report (pdf.gz)
EMICSS annotations (xml)

2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor

EMD-2984

Single-particle
2.2 Å
EMD-2984
3D View Gallery
Deposition: 26/04/2015
Map released: 06/05/2015
Last modified: 14/10/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG)
Sample [1]
Name: Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG)
Number of unique components: 2
Oligomeric state: tetramer
Details: The sample was monodisperse.
Molecular weight
Experimental Theoretical Method
- 465 kDa ProtParam tool: Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A., Protein Identification and Analysis Tools on the ExPASy Server, (in) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005), pp. 571-607
Protein [1]
Name: beta-galactosidase (beta-gal, b-gal)
Number of copies: 1
Oligomeric state: tetramer
UniProtKB PDBe-KB AlphaFold DB
Gene Ontology [1]

Molecular function:
beta-galactosidase activity
Natural source
Organism: Escherichia coli K-12
Cellular location: cytoplasm
Molecular weight
Experimental Theoretical Method
- 465 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Ligand [1]
Name: phenylethyl beta-D-thiogalactopyranoside (PETG)
Number of copies: 4
Oligomeric state: monomer
Details: Molecular weight of PETG is 300.37 Da.
Natural source
Organism: unidentified
Recombinant Expression
Organism Strain Cell Plasmid
- - - -