EMDB EMD-2966

Single particle reconstruction
5.9Å resolution

EM structure of the yeast spliceosomal U4/U6.U5 tri-snRNP

Map released:
Overview of EMD-2966
Source organism: Saccharomyces cerevisiae [4932]
Primary publication:
The architecture of the spliceosomal U4/U6.U5 tri-snRNP
Nguyen THD, Galej WP, Bai X-C, Savva CG, Newman AJ, Scheres SHW, Nagai K
NATURE 523 47-52 (2015)
PMID: 26106855

Function and Biology Details

Sample name: Yeast U4/U6.U5 tri-snRNP
Nucleic acids: U4 snRNA, U5 snRNA, U6 snRNA
Protein: U4/U6.U5 tri-snRNP

Experimental Information Details

Resolution: 5.9Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: CTFFIND3, RELION
Microscope: FEI POLARA 300
Detector: FEI FALCON II (4k x 4k)