EMDB EMD-2561

Single particle reconstruction
21Å resolution

Negative-stain electron microscopy of Hsp104 (HAP form bound to ClpP)

Map released:
Last modified:
Overview of EMD-2561
Source organism: Saccharomyces cerevisiae [4932]
Primary publication:
Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation
Carroni M, Kummer E, Oguchi Y, Clare DK, Wendler P, Sinning I, Kopp J, Mogk A, Bukau B, Saibil HR
elife 3 e02481 (2014)
PMID: 24843029

Function and Biology Details

Sample name: Hsp104 ATPgammaS. HAP variant bound to ClpP.
Protein: Hsp104

Experimental Information Details

Resolution: 21Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C6
Reconstruction software: IMAGIC, Spider
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)