EMDB EMD-2560

Single particle reconstruction
25Å resolution

Negative-stain electron microscopy of E. coli ClpB (BAP form bound to ClpP)

Map released:
Last modified:
Overview of EMD-2560
Source organism: Escherichia coli [562]
Primary publication:
Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation
Carroni M, Kummer E, Oguchi Y, Clare DK, Wendler P, Sinning I, Kopp J, Mogk A, Bukau B, Saibil HR
elife 3 e02481 (2014)
PMID: 24843029

Function and Biology Details

Sample name: ClpB Y503D mutant with ATPgammaS. BAP variant bound to ClpP.
Protein: ClpB

Experimental Information Details

Resolution: 25Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: IMAGIC, Spider
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)