Single particle reconstruction
21Å resolution

Negative-stain electron microscopy of E. coli ClpB mutant E432A (BAP form bound to ClpP)

Map released:
Last modified:
Overview of EMD-2556
Source organism: Escherichia coli [562]
Primary publication:
Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation
Carroni M, Kummer E, Oguchi Y, Wendler P, Sinning I, Kopp J, Mogk A, Bukau B, Saibil HR
elife 3 e02481 (2014)
PMID: 24843029

Function and Biology Details

Sample name: ClpB E432A ATPgammaS. BAP variant bound to ClpP.
Protein: ClpB

Experimental Information Details

Resolution: 21Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: IMAGIC, Spider
Microscope: FEI TECNAI F20
Detector: GATAN K2 (4k x 4k)