Single particle reconstruction
18Å resolution

Negative-stain electron microscopy of E. coli ClpB mutant E432A (BAP form bound to ClpP)

Map released:
Last modified:
Overview of EMD-2555
Source organism: Escherichia coli [562]
Fitted atomic model: 4d2q
Primary publication:
Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation
Carroni M, Kummer E, Oguchi Y, Clare DK, Wendler P, Sinning I, Kopp J, Mogk A, Bukau B, Saibil HR
elife 3 e02481 (2014)
PMID: 24843029

Function and Biology Details

Sample name: ClpB E432A ATPgammaS. BAP variant bound to ClpP.
Protein: ClpB

Experimental Information Details

Resolution: 18Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C6
Reconstruction software: IMAGIC, Spider
Microscope: FEI TECNAI F20
Detector: GATAN K2 (4k x 4k)