EMDB EMD-2540

Single particle reconstruction
18Å resolution

cryo-electron microscopy of microtubule-bound human kinesin-5 motor domain in the ADP state (gold cluster in the N-terminus A9C).

Map released:
Last modified:
Overview of EMD-2540
Source organisms:
Primary publication:
Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family.
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PROC.NAT.ACAD.SCI.USA 111 1837-1842 (2014)
PMID: 24449904

Function and Biology Details

Sample name: 13-protofilament microtubule-bound human kinesin-5 motor domain with ADP bound. A gold cluster is attached to the N-terminus (A9C).
Proteins: alpha tubulin, beta tubulin, Kinesin-5 motor domain

Experimental Information Details

Resolution: 18Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: SPIDER, FREALIGN
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)