EMDB EMD-2533

Single particle reconstruction
9.2Å resolution

Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in the ADP.AlFx state.

Map released:
Last modified:
Overview of EMD-2533
Source organisms:
Fitted atomic models: 4ck6, 4ck7
Primary publication:
Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family.
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PROC.NAT.ACAD.SCI.USA 111 1837-1842 (2014)
PMID: 24449904

Function and Biology Details

Sample name: 13-protofilament microtubule-bound human kinesin-5 motor domain with ADP.AlFx
Proteins: TUBULIN ALPHA-1D CHAIN, TUBULIN BETA-2B CHAIN, Kinesin-5 motor domain

Experimental Information Details

Resolution: 9.2Å
Resolution method: FSC 0.5, semi-independent
Applied symmetry: C1
Reconstruction software: SPIDER, FREALIGN
Microscope: FEI TECNAI F20
Detector: KODAK SO-163 FILM