EMD-2500 Experiments and Validation

Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy

Single particle reconstruction
Overview of EMD-2500
Sample name: Rrp44-Exosome with Streptavidin labeled RNA50
Organisms: Saccharomyces cerevisiae, synthetic construct, Escherichia coli

Map parameters

Recommended contour level: 5.00 (author)
Number of grid points: 90 × 90 × 90
Voxel size: 3.00 × 3.00 × 3.00 Å
Minimum density: -6.853
Maximum density: 17.963
Average density: 0.00
Standard deviation: 1.00

Sample information

Sample name: Rrp44-Exosome with Streptavidin labeled RNA50
Nucleic acid: RNA50
Proteins: Rrp44, Rrp43, Rrp4, Csl4, Rrp45, Rrp46-TAP, Rrp41, Rrp42, Mtr3, Rrp40, Steptavidin

Validation information

Experimental information


Image processing

Applied symmetry: C1
Software: SPIDER
Number of particles: 24913


Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 30.0
Holder model: OTHER
Number of images: 300


Specimen state: particle
Specimen concentration (mg/mL): 1.00
Buffer: 150mM NaCl, 50mM Tris-HCL,1mM DTT
Staining procedure: negatively stained in 2% (w/v) uranyl acetate solution following the standard deep stain procedure on holey-carbon coated EM copper grids covered with a thin layer of continuous carbon
Specimen support: 300 mesh continuous carbon
Apparatus: NONE
Cryogen: NONE