EMDB EMD-2441

Single particle reconstruction
8.6Å resolution

Cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI

Map released:
Last modified:
Overview of EMD-2441
Source organism: Streptomyces griseus [1911]
Fitted atomic model: 4c3g
Primary publication:
Allosteric Regulation of DNA Cleavage and Sequence-Specificity through Run-On Oligomerization.
Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC
STRUCTURE 21 1848-1858 (2013)
PMID: 24055317

Function and Biology Details

Sample name: cryo-EM reconstruction of activated and oligomeric restriction endonuclease SgrAI
Nucleic acid: SgrAI recognition sequence
Protein: SgrAI

Experimental Information Details

Resolution: 8.6Å
Resolution method: FSC 0.143
Applied symmetry: C1
Reconstruction software: Xmipp, and, Frealign
Microscope: FEI TECNAI F20
Detector: DIRECT ELECTRON DE-12 (4k x 3k)