EMDB EMD-2427

Single particle reconstruction
23Å resolution

Negative stain Electron Microscopy of BG505 SOSIP.664 gp140 in complex with PGV04

Map released:
Overview of EMD-2427
Source organisms:
Related EM entry by publication: EMD-5723
Primary publication:
A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies.
Sanders RW, Derking R, Cupo A, Julien JP, Yasmeen A, de Val N, Kim HJ, Blattner C, Torrents A, Korzun J, Golabek M, de los Reyes K, Ketas TJ, van Gils MJ, King CR, Wilson IA, Ward AB, Klasse PJ, Moore JP
PLOS PATHOG. 9 e1003618-e1003618 (2013)
PMID: 24068931

Function and Biology Details

Sample name: HIV spike protein 664G construct in complex with Fab fragment of PGV04 monoclonal antibody
Proteins: HIV spike protein BG505 SOSIP.664, PGV04 FAB

Experimental Information Details

Resolution: 23Å
Resolution method: FSC 0.5
Applied symmetry: C3
Reconstruction software: EMAN2, EMAN1
Microscope: FEI TECNAI F20
Detector: TVIPS TEMCAM-F416 (4k x 4k)