EMD-2416

Single-particle
30.0 Å
EMD-2416
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Deposition: 17/07/2013
Map released: 23/10/2013
Last modified: 23/10/2013
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EMD-2416
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Negative staining 3D-EM of the HA complex of Botulinum toxin type A

EMD-2416

Single-particle
30.0 Å
EMD-2416
3D View Gallery
Deposition: 17/07/2013
Map released: 23/10/2013
Last modified: 23/10/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 7.6
Details: 50mM NaCl, 20mM Tris-HCL
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein floated on 1% w/v uranyl formate for 30 seconds
Grid
Details: 300 mesh copper grid with carbon support, glow discharged in vacuumed air
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.5 µm - 3.6 µm
Calibrated magnification: 70000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 70,000 times magnification., Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: CCD
Detector model: GENERIC TVIPS (4k x 4k)
Number of real images: 62
Average electron dose per image: 40 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: Image processing was done using EMAN2.The particles were selected by a semi-automatic selection using e2boxer.
Final reconstruction
Resolution: 30.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3746
Algorithm: OTHER
Details: C3 symmetry was imposed in refinement
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
EMAN2 - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Negative staining 3D-EM of the HA complex of Botulinum toxin A
Details: ::::EMDATABANK.org::::EMD-2416::::
⬡ Geometry
X Y Z
Dimensions 120 120 120
Origin -60 -60 -60
Spacing 120 120 120
Voxel size 4.28 Å 4.28 Å 4.28 Å
Contour list
Primary Level Source
True 0.55 AUTHOR