EMDB EMD-2416

Single particle reconstruction
30.0Å resolution

Negative staining 3D-EM of the HA complex of Botulinum toxin type A

Map released:
Overview of EMD-2416
Source organism: Clostridium botulinum [1491]
Related EM entry by publication: EMD-2417
Primary publication:
Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.
Lee K, Gu S, Jin L, Le TT, Cheng LW, Strotmeier J, Kruel AM, Yao G, Perry K, Rummel A, Jin R
PLOS PATHOG. 9 e1003690-e1003690 (2013)
PMID: 24130488

Function and Biology Details

Sample name: The trimeric HA complex of Botulinum toxin type A
Proteins: Botulinum toxin type A HA17, Botulinum toxin type A HA33, Botulinum toxin type A HA70

Experimental Information Details

Resolution: 30.0Å
Resolution method: FSC 0.5
Applied symmetry: C3
Reconstruction software: EMAN2
Microscope: FEI TECNAI F20
Detector: GENERIC TVIPS (4k x 4k)