EMD-2397

Single-particle
10.0 Å
EMD-2397
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Deposition: 11/06/2013
Map released: 05/02/2014
Last modified: 19/02/2014
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2397
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4c0u summary report (pdf.gz) Map and 4c0u FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM reconstruction of enterovirus 71 in complex with a neutralizing antibody E18

EMD-2397

Single-particle
10.0 Å
EMD-2397
3D View Gallery
Deposition: 11/06/2013
Map released: 05/02/2014
Last modified: 19/02/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Buffer
pH: 8.0
Details: 10 mM Tris-HCl, pH 8.0, 0.5 M NaCl, and 1 mM EDTA
Grid
Details: Quantifoin R1.2/1.3 Holey carbon 200Mesh
Vitrification
Cryogen name: ETHANE
Chamber humidity: 70%
Chamber temperature: 100 K
Instrument: HOMEMADE PLUNGER
Method: Blot for 2 second before plunging
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 0.00075 µm - 0.0045 µm
Nominal magnification: 50000.0
Calibrated magnification: 4950.0
Specimen holder model: OTHER
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Temperature
Minimum: 95 K
Average: 119 K
Maximum: 120 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Number of real images: 112
Average electron dose per image: 18 e/Å2
Bits per pixel: 16.0
Image processing [1]
Details: Particles were boxed with e2boxer.py
Final reconstruction
Resolution: 10.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8335
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Eman, eman2 - -
Final 2D classification
Number of classes: 122
CTF correction
Details:CTF determined for all particles from one film, phase flip
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of EV71 E18 Fab complex
Details: ::::EMDATABANK.org::::EMD-2397::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin -100 -100 -100
Spacing 200 200 200
Voxel size 2.48 Å 2.48 Å 2.48 Å
Contour list
Primary Level Source
True 1.0 AUTHOR