EMD-2386 Experiments and Validation

Cryo electron microscopy of yeast INO80

Single particle reconstruction
Overview of EMD-2386
Sample name: S. cerevisiae INO80 chromatin remodeler
Organism: Saccharomyces cerevisiae
Related EM entry by publication: EMD-2385

Map parameters

Minimum density: -0.001
Maximum density: 0.002
Average density: -0.00
Standard deviation: 0.00
Recommended contour level: 0.00 (emdb)

Sample information

Sample name: S. cerevisiae INO80 chromatin remodeler
Protein: INO80

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Spider, EMAN2, Sparx
Number of particles: 386988

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 20.0
Nominal CS (mm): 2.70
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 95