EMD-2385 Experiments and Validation

Negative stain electron microscopy of yeast INO80

Single particle reconstruction
Overview of EMD-2385
Sample name: S. cerevisiae INO80 chromatin remodeler
Organism: Saccharomyces cerevisiae
Related EM entry by publication: EMD-2386

Map parameters

Minimum density: -0.058
Maximum density: 0.106
Average density: -0.00
Standard deviation: 0.003
Recommended contour level: 0.003 (emdb)

Sample information

Sample name: S. cerevisiae INO80 chromatin remodeler
Protein: INO80

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Spider, EMAN2, Sparx
Number of particles: 14386

Imaging

Session
Microscope model: OTHER
Electron source: LAB6
Nominal CS (mm): 2.20
Holder model: SIDE ENTRY, EUCENTRIC

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: NONE
Cryogen: NONE