EMDB EMD-2361

Single particle reconstruction
23Å resolution

Electron cryo-EM of the full-length Thermus thermophilus DNA gyrase in complex with a 155bp DNA and ciprofloxacin

Map released:
Last modified:
Overview of EMD-2361
Source organisms:
Related EM entry by publication: EMD-2360
Primary publication:
Structural insight into negative DNA supercoiling by DNA gyrase, a bacterial type 2A DNA topoisomerase.
Papillon J, Menetret JF, Batisse C, Helye R, Schultz P, Potier N, Lamour V
NUCLEIC ACIDS RES. 41 7815-7827 (2013)
PMID: 23804759

Function and Biology Details

Sample name: DNA-bound complex of Thermus thermophilus DNA gyrase with a 155bp DNA in presence of ADPNP and ciprofoxacin.
Nucleic acid: linear DNA
Protein: DNA gyrase

Experimental Information Details

Resolution: 23Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN2
Microscope: FEI TECNAI F30
Detector: FEI EAGLE (4k x 4k)