EMD-2359 Experiments and Validation

Structure and conformational variability of the Mycobacterium tuberculosis fatty acid synthase multienzyme complex

Single particle reconstruction
Overview of EMD-2359
Sample name: M. tuberculosis type-I fatty acid synthase
Organism: Mycobacterium tuberculosis
Related EM entry by publication: EMD-2357, EMD-2358

Map parameters

Recommended contour level: 2.10 (author)
Number of grid points: 200 × 200 × 200
Voxel size: 2.28 × 2.28 × 2.28 Å
Minimum density: -2.63
Maximum density: 11.121
Average density: 0.00
Standard deviation: 1.00

Sample information

Sample name: M. tuberculosis type-I fatty acid synthase
Protein: Type-I fatty acid synthase

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: EMAN2, SPARX
Number of particles: 3357

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Nominal magnification: 59000.0
Defocus max (nm): 4,500.00
Defocus min (nm): 1,800.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: ZEISS SCAI

Specimen

Preparation
Specimen state: particle
Specimen support: Quantifoils copper grid
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE