EMD-2358 Experiments and Validation

Structure and conformational variability of the Mycobacterium tuberculosis fatty acid synthase multienzyme complex

Single particle reconstruction
Overview of EMD-2358
Sample name: M. tuberculosis type-I fatty acid synthase
Organism: Mycobacterium tuberculosis
Fitted atomic model: 4v8v
Related EM entry by publication: EMD-2357, EMD-2359

Map parameters

Recommended contour level: 1.80 (emdb)
Number of grid points: 200 × 200 × 200
Voxel size: 2.28 × 2.28 × 2.28 Å
Minimum density: -2.852
Maximum density: 10.361
Average density: 0.00
Standard deviation: 1.00

Sample information

Sample name: M. tuberculosis type-I fatty acid synthase
Protein: Type-I fatty acid synthase

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: EMAN2, SPARX
Number of particles: 4337

Fitting

Initial atomic model: 3zen
Used chains: D, E, F
Fitting protocol: rigid body
Refinement space: REAL

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Nominal magnification: 59000.0
Defocus max (nm): 4,500.00
Defocus min (nm): 1,800.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: ZEISS SCAI

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.00
Specimen support: Quantifoils copper grid
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE