EMDB EMD-2327

Single particle reconstruction
15.9Å resolution

Visualizing GroEL/ES in the Act of Encapsulating a Non-Native Substrate Protein

Map released:
Last modified:
Overview of EMD-2327
Source organisms:
Fitted atomic model: 3zq1
Related EM entry by publication: EMD-2325, EMD-2326
Primary publication:
Visualizing GroEL/ES in the act of encapsulating a folding protein.
Chen D-H, Madan D, Weaver J, Lin Z, Schroder GF, Chiu W, Rye HS
CELL(CAMBRIDGE,MASS.) 153 1354-1365 (2013)
PMID: 23746846

Function and Biology Details

Sample name: Non-native RuBisCO substrate protein encapsulated inside the cavity of GroEL capped by GroES with the assistance of nucleotide ATP
Ligand: Adenosine triphosphate
Proteins: GroEL-D398A, GroES, Ribulose-1,5-bisphosphate carboxylase oxygenase

Experimental Information Details

Resolution: 15.9Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: EMAN1
Microscope: JEOL 3200FSC
Detector: GENERIC GATAN