EMD-2321 Function and Biology

The bacterial DnaC helicase loader is a DnaB ring breaker

Single particle reconstruction
25Å resolution
Overview of EMD-2321
Sample name: E. coli DnaB helicase bound to the DnaC loading factor
Number of unique components: 2
Oligomeric state: One homohexamer of DnaB binds to one homohexamer of DnaC
Theoretical weight (MDa): 0.48

DnaB replicative helicase [Escherichia coli]

Protein type
Name: DnaB replicative helicase
Alternative name: Replicative DNA helicase
Theoretical weight (MDa): 0.052
Number of copies: 6
Oligomeric state: hexamer
UniProt: P0ACB0
InterPro: IPR007692
Expression system
Expression system: Escherichia coli

DnaC helicase loading factor [Escherichia coli]

Protein type
Name: DnaC helicase loading factor
Alternative name: DNA replication protein DnaC
Theoretical weight (MDa): 0.028
Number of copies: 6
UniProt: P0AEF0
Expression system
Expression system: Escherichia coli