Single particle reconstruction
20Å resolution

Single particle cryo-microscopy reconstruction of the isolated Manduca sexta V1 domain

Map released:
Last modified:
Overview of EMD-2318
Source organism: Manduca sexta [7130]
Related in-frame EM entry: EMD-2317
Related EM entry by publication: EMD-2317
Primary publication:
Subunit positioning and stator filament stiffness in regulation and power transmission in the V1 motor of the Manduca sexta V-ATPase.
Muench SP, Scheres SH, Huss M, Phillips C, Vitavska O, Wieczorek H, Trinick J, Harrison MA
J.MOL.BIOL. 426 286-300 (2014)
PMID: 24075871

Function and Biology Details

Sample name: M. sexta isolated V1 domain without subunit C
Protein: Vacuolar ATPase isolated V1 domain

Experimental Information Details

Resolution: 20Å
Resolution method: Gold standard FSC curve, gold-standard
Applied symmetry: C1
Reconstruction software: Relion
Microscope: FEI TECNAI F20
Detector: GENERIC GATAN (4k x 4k)