EMDB EMD-2307

Single particle reconstruction
12.7Å resolution

Structural visualization of key steps in human transcription initiation

Map released:
Last modified:
Overview of EMD-2307
Source organism: Homo sapiens [9606]
Related EM entries by publication: EMD-2309, EMD-2308, EMD-2305, EMD-2304, EMD-2306
Primary publication:
Structural visualization of key steps in human transcription initiation.
He Y, Fang J, Taatjes DJ, Nogales E
NATURE 495 481-486 (2013)
PMID: 23446344

Function and Biology Details

Sample name: TBP-TFIIA-TFIIB-DNA-Pol II-TFIIF-TFIIE (open)
Nucleic acid: Core promoter DNA
Proteins: TATA-box-binding protein, General transcription factor IIA, General transcription factor IIB, General transcription factor IIF, General transcription factor IIE, DNA-directed RNA polymerase II

Experimental Information Details

Resolution: 12.7Å
Resolution method: FSC 0.143
Applied symmetry: C1
Reconstruction software: EMAN2, SPARX
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)