EMD-2300 Experiments and Validation

Negative stain EM structure of the PTC3 holotoxin complex (TcdA1, TcdB2, TccC3) in pore state

Single particle reconstruction
Overview of EMD-2300
Sample name: PTC3 (TcdA1, TcdB2, TccC3)
Organism: Photorhabdus luminescens
Related EM entries by publication: EMD-2301, EMD-2299, EMD-2298, EMD-2297

Map parameters

Minimum density: -0.036
Maximum density: 0.073
Average density: 0.001
Standard deviation: 0.005
Recommended contour level: 0.008 (author)

Sample information

Sample name: PTC3 (TcdA1, TcdB2, TccC3)
Proteins: TcdA1, TcdB2, TccC3

Validation information

Experimental information

 

Image processing

Applied symmetry: C5
Software: Sparx
Number of particles: 317
Reconstruction protocol: Projection matching

Imaging

Session
Electron source: LAB6
Nominal CS (mm): 3.40
Nominal magnification: 50000.0
Defocus max (nm): 2.00
Defocus min (nm): 1.20
Holder model: JEOL
Acquisition
Number of images: 104

Specimen

Preparation
Specimen state: particle
Buffer: 50 mM CAPS, 100 mM NaCl, 0.05% Tween-20, 5% glycerol
Staining procedure: Grids with adsorbed Protein floated on 0.07% Uranyl Formate for 45 sec.
Specimen support: glow discharged 400 mesh copper grids with thin carbon support
Vitrification
Apparatus: NONE
Cryogen: NONE