Single particle reconstruction
15Å resolution

Second 3D model of the MjHSP16.5 mutant R107G at 60 degree Celsius by CryoEM.

Map released:
Last modified:
Overview of EMD-2295
Source organism: Methanocaldococcus jannaschii [2190]
Related EM entries by publication: EMD-2288, EMD-2289, EMD-2293, EMD-2292, EMD-2291, EMD-2290, EMD-2294
Primary publication:
Changes in the quaternary structure and function of MjHSP16.5 attributable to deletion of the IXI motif and introduction of the substitution, R107G, in the -crystallin domain.
Quinlan RA, Zhang Y, Lansbury A, Williamson I, Pohl E, Sun F
Philos Trans R Soc Lond B Biol Sci 368 20120327-20120327 (2013)
PMID: 23530263

Function and Biology Details

Sample name: R107G mutation of small heat shock protein (sHSP) HSP16.5 from Methanocaldococcus jannaschii (MjHSP16.5)
Protein: MjHSP16.5 R107G mutation

Experimental Information Details

Resolution: 15Å
Resolution method: FSC 0.5
Applied symmetry: O
Reconstruction software: EMAN1
Detector: GATAN ULTRASCAN 4000 (4k x 4k)