Single particle reconstruction
48.0Å resolution

negative stain single-particle reconstruction of conformation III of the Ltn1 E3 ubiquitin Ligase

Map released:
Last modified:
Overview of EMD-2250
Source organism: Saccharomyces cerevisiae [4932]
Primary publication:
Single-particle EM reveals extensive conformational variability of the Ltn1 E3 ligase.
Lyumkis D, Doamekpor SK, Bengtson MH, Lee JW, Toro TB, Petroski MD, Lima CD, Potter CS, Carragher B, Joazeiro CA
PROC.NAT.ACAD.SCI.USA 110 1702-1707 (2013)
PMID: 23319619

Function and Biology Details

Sample name: conformational snapshot III of Ltn1
Protein: Ltn1

Experimental Information Details

Resolution: 48.0Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: Spider, Appion
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)